FUNGAL FLORA OF HORDEUM VULGARE L. AND EFFECT OF ULTRA VIOLET C RADIATIONS ON IT
Keywords:Fungal flora, Storage period, Ultra violet radiations, Saprophytic and pathogenic fungi
Twenty four fungal species from 13 genera were identified from 12 samples of barley collected from different localities of Pakistan. These fungi were detected by three methods namely, blotter, agar plate and deep freezing methods described by International Seed Testing Association (ISTA). The incidence of fungal flora was comparatively higher in agar plate method as compared to blotter and deep freezing methods. Surface sterilized of seed reduced the incidence of saprophytic fungi while, the incidence of saprophytic fungi was higher in non- sterilized condition. Aspergillus species were observed in all samples tested particularly Aspergillus niger. The most frequently isolated and identified fungi were Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Rhizopus stolonifer, Mucor sp. and Trichoderma harzianum. Barley seeds sample collected from Kashmir showed maximum number of fungi were further subjected to treat with ultra violet C (UVC) for different time period. Results showed that incidence of fungi greatly reduced after 30 and 60 days of storage. However, A. alternata and A. fumigatus have no effect after 60 days of storage at room temperature.
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